gene chip arraystar lncrna v3.0 microarray  (Arraystar inc)

Journal: Frontiers in Oncology

Article Title: SNHG9, a Papillary Thyroid Cancer Cell Exosome-Enriched lncRNA, Inhibits Cell Autophagy and Promotes Cell Apoptosis of Normal Thyroid Epithelial Cell Nthy-ori-3 Through YBOX3/P21 Pathway

doi: 10.3389/fonc.2021.647034

Figure Lengend Snippet: Identification and expression of PTC cell exosome-enriched lncRNA SNHG9. (A) High-throughput screening identification of PTC associated exosome lncRNAs. SNHG9 is PTC cell exosome-enriched lncRNA in TPC-1 and K-1 cells compared with Nthy-ori-3 cell. (B, C) Validation of SNHG9 overexpression in both TPC-1 and K-1 cells and their respective exosomes compared with Nthy-ori-3 cell and its exosome by qPCR. (D) Coregulation network of SNHG9 with mRNA/miRNA. SNHG9 had an interaction with autophagy related molecules. (E) Gene ontology enrichment analysis showed the highest regulation scores in autophagy and apoptosis. (F) KEGG-pathway-weighted analysis showed SNHG9 mainly targeted apoptosis and autophagy pathways. (G) SNHG9 in the PTC cell supernatant mainly derived from cell exosomes. QPCR showed significantly lower SNHG9 expression level in supernatant treated with Rnase and Triton compared with supernatant treated with only Rnase and control group. (H) QPCR confirmed no SNHG9 expression in cell supernatants after exosome extraction. (I) SNHG9 expression level between tumor and normal tissues in 70 PTC patients from FUSCC. The results were normalized to β-actin mRNA level. (J) Waterfall plot showed the distribution of SNHG9 expression level in each PTC patients from FUSCC. ***P < 0.001, data were pooled from three independent experiments. FUSCC, Fudan University Shanghai Cancer Center; PTC, papillary thyroid cancer.

Article Snippet: Next, we used the Arraystar Human LncRNA Array v2.0 gene chip to compare expression profile data of lncRNAs in Nthy-ori-3, TPC-1 and K-1 cells and their respective exosomes.

Techniques: Expressing, High Throughput Screening Assay, Biomarker Discovery, Over Expression, Derivative Assay, Control, Extraction

Journal: Aging (Albany NY)

Article Title: Long non-coding RNA Loc490 inhibits gastric cancer cell proliferation and metastasis by upregulating RNA-binding protein Quaking

doi: 10.18632/aging.103876

Figure Lengend Snippet: Low expression of Loc490 in GC and lymph node metastasis tissue. ( A ) RNA quality testing (C: GC tissue; L: lymph node metastasis tissue; N: normal tissue adjacent to GC); ( B ) Screening for differentially expressed lncRNAs using Agilent Human LncRNA chip technology in normal tissues adjacent to GC, primary GC tissues and lymph node metastasis tissues; ( C ) Loc490 expression was significantly lower in lymph node metastases than in normal tissues adjacent to GC and in primary GC tissue (the other selected genes are shown in the article); ( D ) qRT-PCR results demonstrating Loc490 expression in 28 normal tissues adjacent to GC, primary GC tissues and lymph node metastasis tissues; ( E ) Expression of QKI mRNA in 28 normal tissues adjacent to GC, primary GC tissues and lymph node metastases; ( F ) Correlation analysis demonstrating that Loc490 expression was significantly positively correlated with QKI mRNA expression. Data represent the mean and SD from three experiments. **p < 0.01, ***p < 0.001.

Article Snippet: We used an Agilent Human LncRNA gene chip to compare the lncRNA and mRNA levels in GC and metastatic lymph node tissues with those in normal tissues adjacent to the GC.

Techniques: Expressing, Quantitative RT-PCR